Author: Salitha & Kulkarni Surendra Gopal
Citation: Gopal, Kulkarni Surendra. "Comparative assessment of soil bacterial diversity as influenced by novel biofertilizer application in nutmeg using metagenomic analysis." Biologia 80.7 (2025): 1835-1844.
Abstract:
https://link.springer.com/article/10.1007/s11756-025-01944-1
Nutmeg (Myrstica fragrans Houtt.), an important export-oriented crop from India, is a nutrient exhaustive tree spice. Biofertilization of nutmeg could reduce the chemical dependency of soils and various changes in the soil physico-chemical properties and microbiological parameters. The aim of this study was to assess the soil biodiversity in the initial soil sample and final soil sample (Staphylococcus equorum + Bacillus velezensis + Staphylococcus epidermidis + 50% recommended dose of fertilizers based on soil test data). In the study, the total soil DNA of initial soil and final soil were extracted and subjected to a partial 16S rRNA gene sequencing using V3F and V4R primers. The differences in prokaryotic diversity were revealed through metagenomics analysis. The study revealed that bulk and final soil samples had several bacterial phyla in common, varying in their percentage composition. However, Proteobacteria, Actinobacteria and Acidobacteria were the most dominant bacterial phyla in both the soil samples. In the soils, these phyla were globally widespread in distribution. Actinoallomurus and Sphingomicrobium were the major dominant bacterial genus found in the initial soil sample and final soil sample respectively. Diversity analysis indices (Shannon, Simpson) revealed that bacterial composition was slightly different in both the soil samples (initial and final). Unclassified bacterial groups were present in both the soil samples revealing the presence of potential novel bacterial species. The bacterial community and composition varied between the initial soil sample and final soil samples. Proper knowledge on the soil and plant microbiome could help in agricultural sustainability with rhizosphere management practices.
Author: Salitha & Kulkarni Surendra Gopal
Citation: Gopal, Kulkarni Surendra. "Comparative assessment of soil bacterial diversity as influenced by novel biofertilizer application in nutmeg using metagenomic analysis." Biologia 80.7 (2025): 1835-1844.
Abstract:
https://link.springer.com/article/10.1007/s11756-025-01944-1
Nutmeg (Myrstica fragrans Houtt.), an important export-oriented crop from India, is a nutrient exhaustive tree spice. Biofertilization of nutmeg could reduce the chemical dependency of soils and various changes in the soil physico-chemical properties and microbiological parameters. The aim of this study was to assess the soil biodiversity in the initial soil sample and final soil sample (Staphylococcus equorum + Bacillus velezensis + Staphylococcus epidermidis + 50% recommended dose of fertilizers based on soil test data). In the study, the total soil DNA of initial soil and final soil were extracted and subjected to a partial 16S rRNA gene sequencing using V3F and V4R primers. The differences in prokaryotic diversity were revealed through metagenomics analysis. The study revealed that bulk and final soil samples had several bacterial phyla in common, varying in their percentage composition. However, Proteobacteria, Actinobacteria and Acidobacteria were the most dominant bacterial phyla in both the soil samples. In the soils, these phyla were globally widespread in distribution. Actinoallomurus and Sphingomicrobium were the major dominant bacterial genus found in the initial soil sample and final soil sample respectively. Diversity analysis indices (Shannon, Simpson) revealed that bacterial composition was slightly different in both the soil samples (initial and final). Unclassified bacterial groups were present in both the soil samples revealing the presence of potential novel bacterial species. The bacterial community and composition varied between the initial soil sample and final soil samples. Proper knowledge on the soil and plant microbiome could help in agricultural sustainability with rhizosphere management practices.