Plant growth-promoting bacteria (PGPB) have many uses in agriculture and environmental management. They work as natural biostimulants and biofertilizers, helping crops grow better and yield more in sustainable ways.
This study focused on isolating three active bacterial strains from dry (arid) rhizosphere soil to be used as biofertilizers. Their growth-promoting abilities were tested in the lab (in vitro) and then applied to evaluate their effects on pepper plant growth and yield.
The three bacterial isolates—P21, P22-1, and P58—showed strong production of indole-3-acetic acid (IAA), a plant growth hormone. They produced 154.47 µg/mL, 155.03 µg/mL, and 188.65 µg/mL of IAA, respectively. They also produced notable amounts of ammonia after 72 hours of growth in a peptone water medium. Isolate P21 produced the most ammonia (17.38 μmol/mL), while P22-1 and P58 produced slightly less (15.47 and 15.92 μmol/mL), with no significant difference between them.
The phosphate (P) solubilization efficiency of these isolates was 18.7% for P21, 64% for P22-1, and 54% for P58 compared to an un-inoculated control medium. Molecular identification using 16S rRNA sequencing confirmed their species as Pseudomonas alkylphenolica strain P21 (PX257452), Pantoea agglomerans strain P22-1 (PX257453), and Pantoea brenneri strain P58 (PX257454).
Plant growth-promoting bacteria (PGPB) have many uses in agriculture and environmental management. They work as natural biostimulants and biofertilizers, helping crops grow better and yield more in sustainable ways.
This study focused on isolating three active bacterial strains from dry (arid) rhizosphere soil to be used as biofertilizers. Their growth-promoting abilities were tested in the lab (in vitro) and then applied to evaluate their effects on pepper plant growth and yield.
The three bacterial isolates—P21, P22-1, and P58—showed strong production of indole-3-acetic acid (IAA), a plant growth hormone. They produced 154.47 µg/mL, 155.03 µg/mL, and 188.65 µg/mL of IAA, respectively. They also produced notable amounts of ammonia after 72 hours of growth in a peptone water medium. Isolate P21 produced the most ammonia (17.38 μmol/mL), while P22-1 and P58 produced slightly less (15.47 and 15.92 μmol/mL), with no significant difference between them.
The phosphate (P) solubilization efficiency of these isolates was 18.7% for P21, 64% for P22-1, and 54% for P58 compared to an un-inoculated control medium. Molecular identification using 16S rRNA sequencing confirmed their species as Pseudomonas alkylphenolica strain P21 (PX257452), Pantoea agglomerans strain P22-1 (PX257453), and Pantoea brenneri strain P58 (PX257454).
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